Shampa Chatterjee, Ph.D.

Research Assistant Professor of Physiology

Institute for Environmental Medicine
1 John Morgan Building
3620 Hamilton Walk
Philadelphia. PA. 19104

 

Tel: 215-898-9821/9093

Fax: 215-898-0868

Email: shampac@mail.med.upenn.edu

 

Research Interests

Dr. Chatterjee’s research involves understanding how cells transduce physical forces or

 

 

 

environmental cues into biochemical signals. In this direction, endothelial cells that line the blood vessels are unique by virtue of their location that allows them to constantly experience changes in blood flow. Alterations in shear initiate a signaling cascade that is first triggered by mechanosensitive elements on endothelial cells. Our interest is in identification of these elements; besides an understanding of endothelial signaling, these elements could be targeted to modulate responses to start or stop of flow. Among other mechanosensitive elements, we have identified a KATP channel in the pulmonary vasculature that plays a role in sensing alterations of flow. We also seek answers to whether endothelial signaling by other physical forces is also initiated through this (and other ion channels). In addition we are also exploring the role of mechanosensing in modulating endothelial responses and phenotype.

 

FF                                                  Principal Investigator/Program Director (Last, first, middle): Chatterjee

BIOGRAPHICAL SKETCH

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NAME                     Shampa Chatterjee, Ph.D.

 

POSITION TITLE           Research Assistant Professor

EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training)

INSTITUTION AND LOCATION

DEGREE

(in applicable)

YEAR(s)

FIELD OF STUDY

 

Indian Institute of Technology, Bombay-76, India

 

 

Ph.D.

 

 

1991-97

 

 

Chemistry

 

RESEARCH AND PROFESSIONAL EXPERIENCE::  Concluding with present position, list, in chronological order, previous employment, experience, and honors. Include present membership on any Federal Government public advisory committee. List, in chronological order, the titles, all authors, and complete references to all publications during the past three years and to representative earlier publications pertinent to this application. If the list of publications in the last hree years exceeds two pages, select the most pertinent publications.  DO NOT EXCEED TWO PAGES.

RESEARCH AND/OR PROFESSIONAL EXPERIENCE

1989-1990             Lecturer, Department of Chemistry (Supervisor, Dr. T. Padmanaban), SIES College University of Bombay, Bombay, India

1991-1997             PhD Fellow, Department of Chemistry (Supervisor, Professor T.S. Srivastava),

                              Indian Institute of Technology, Bombay, India

1997-1999            UNESCO Research Trainee, GBF (Supervisor, Professor G. Wolf), Braunschweig, Germany, Post-doctoral Fellow, Institute for Medical Neurobiology (Supervisor, Professor G. Wolf),

                              Magdeburg, Germany

1999-2002             Post-doctoral Fellow, Institute for Environmental Medicine (Supervisor, Dr. Aron B. Fisher).

2002-2005       Research Associate, Institute for Environmental Medicine,University of   Pennsylvania School  of Medicine, Philadelphia, PA

2006-             Research Assistant Professor, Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA 

 

AWARDS

Julius Comroe, Jr. Award, American Physiological Society 2002.

Caroline tum Suden Professional Excellence Award, American Physiology Society, 2000

UNESCO International Training Programme Fellowship-97, Braunschweig, Germany

CSIR Research Fellowship Award, Ministry of Human Resources, New Delhi, India

National Talent Search Scholarship Award, Department of Education, New Delhi, India

 

 

 

 

PUBLICATIONS:  Recent Articles

 

Chattoraj, D.K., Biswas, S.C., Mahapatra, P.K. and Chatterjee, S. Standard  free energies of binding of  solute to proteins  in aqueous  medium. Part 2. Analysis of data obtained from equilibrium  dialysis and isopiestic experiments.Biophysical Chemistry, 77 (1999) 9-25.

Chatterjee, S., Noack, H., Possel, H., Keilhoff, G. and Wolf, G. Glutathione Levels in Primary Glial Cultures: Monochlorobimane Provides Evidence of Cell Type Specific Distribution. Glia, 27:152-161, 1999.

Chatterjee, S., Possel. H., Srivastava, T.S. and Wolf, G. et al. Photodynamic effects of meso-tetrakis[4-carboxymethyleneoxy)phenyl]porphyrin on isolated Sarcoma 180 ascites mitochondria. Photochem. Photobiol. B:Biol 50:79-87, 1999.

Jiang,L., Yang, Y.,  Chatterjee, S. and Yang, S., The expression of ProUK in Escherichia coli : the vgb promoter  replaces  IPTG and  coexpression  of  argU compensates for rare codons in a hypoxic induction model. BioSci Biotech Biochem 63:2097-2101, 1999.

Chatterjee, S. and Srivastava, T.S. Spectral Investigations of the interactions of some water soluble porphyrins with bovine serum albumin. J. Porphyrins & Phthalocyanines, 4:147-157, 2000.

Chatterjee, S., Noack, H., Possel, H. and Wolf, G., Induction of nitric oxide synthase in glial cultures: effect on glutathione levels. Glia, 29:98-101, 2000.

Noack, H., Possel, H., Chatterjee, S., Keilhoff, G. and Wolf, G., Nitrosative stress in primary glial cultures after induction of the inducible isoform of nitric oxide synthase (i-NOS). Toxicology, 148:133-142, 2000.

Chatterjee, S., Al-Mehdi AB, Levitan I, Stevens T, Fisher AB. Shear stress increases expression of a KATP channel in rat and bovine pulmonary vascular endothelial cells. Am J Physiol Cell Physiol.  285: C959-C967, 2003.

Milavonova, T., Manevich, Y., Haddad, A., Chatterjee, S., Moore, J.S., Fisher, A.B. Endothelial cell  proliferation associated with abrupt reduction in shear stress is dependent on reactive oxygen species. Antiox. Redox Signal.  6: 245-258, 2004.

Wei, Z., Manevich, Y., Al-Mehdi, A.B., Chatterjee, S. And Fisher, A.B. Ca2+ flux through voltage gated channels with flow cessation in pulmonary microvascular endothelial cells. Microcirculation 11:517-526, 2004.

Chatterjee, S. and Fisher, A.B. ROS to the rescue Am J Physiol Lung Cell Mol Physiol. 287:L704-L705, 2004. 

Matsuzaki, I., Chatterjee, S., DeBolt, K., Manevich, Y., Zhang, Q., Fisher, A.B. Membrane Depolarization and  NADPH Oxidase Activation in Aortic Endothelium During Ischemia Reflect Altered Mechanotransduction.

     Am J Physiol Heart Circ Physiol. 288: H336-343, 2005.

Zhang, Q., Matsuzaki, I., Chatterjee, S., Fisher, A.B.  Activation of Endothelial NADPH Oxidase during Normoxic Lung Ischemia is KATP Channel Dependent Am J Physiology Lung Cell Mol Physiol. 289:

L954-61, 2005.

 Milovanova, T., Chatterjee, S., Moore, J., Manevich, Y., Fisher, A.B. Lung Endothelial Cell Proliferation with decreased shear stress is mediated by reactive oxygen species. Am J Physiol Cell Physiol. 290: C66-76,

2006.

Chatterjee, S., Levitan, I., Wei, Z., Fisher, A.B. KATP channels are an important component of the shear sensing  mechanism in the pulmonary microvasculature. Accepted for publication (Mircocirculation)  

S. Chatterjee and A.B. Fisher, Role of ischemia reperfusion injury in graft rejection. in “Immunobiology of Organ Transplantation’’ Eds. Wilkes, D.S and Burlingham, W.J. Kluwer Academic  Press. pp545.

S. Chatterjee and A. B. Fisher, Oxidative Stress and Cancer, in “Phytochemicals in Cancer Chemoprevention. Eds. Bagchi, D. and Preuss, H. CRC Press 2004.

 

ABSTRACTS (past 3 years)

 

Chatterjee, S., Al Mehdi, A.B., Levitan, I. and Fisher, A.B.  Shear stress increases KATP channel expression and activity in pulmonary endothelial cells.  FASEB J.  A438, 16, 2002.

Chatterjee, S., Levitan, I., Al-Mehdi, A.B., Manevich, Y. and Fisher, A.B.  A KATP channel on the microvascular endothelial cells is involved in membrane depolarization and oxidant generation with change in shear (International Conference on the role of free radicals in health and disease and IInd annual conference of Society of Free radical research (India), Lucknow, India, Feb 10-12, 2003.  

Chatterjee, S., Levitan, I. and Fisher, A.B., A KATP channel on pulmonary microvascular endothelial cells is shear stress sensitive. Am. J. Res. Crit. Care Med. 2003, 167, A796.

Fisher, A.B., Milovanova, T., Manevich, Y., Chatterjee, S., Moore, J.S. Endothelial cell associated with abrupt reduction in shear stress is dependent on reactive oxygen species, 43rd Annual Meeting of American Society of Cell Biology, San Francisco, Dec 14-17, 2003.

Chatterjee, S., Levitan, I., Al-Mehdi, A.B., Manevich, Y. and Fisher, A.B.,

Shear stress, KATP channel and ischemic responses in the pulmonary microvasculature.  International Conference on the role of free radicals in health and disease and IInd annual conference of Society of Free radical research (India), Chidambaram, India, Feb 10-12, 2004.  

Chatterjee, S., Levitan, I., Wei, Z., Al-Mehdi, A.B., Seino, S., Miki, T., Milovanova, T., Hawkins, B., Matsuzaki, I., Feinstein, S. and Fisher, A.B. KATP channel in the pulmonary microvasculature is an important component of the shear stress sensing mechanism. FASEB J. 2004, 18, A329. 

Zhang, Q., Matsuzaki, I., Chatterjee, S., Fisher, A.B. Mice with Kir6.2-knockout are resistant to endothelial cell reactive oxygen (ROS) generation during normoxic lung ischemia. FASEB J. 2004, 18, A329.

Milovanova, T., Manevich, Y., Chatterjee, S., Kotelnikova, I., DeBolt, K., Muniswamy, M., Moore, J. and Fisher, A.B.  Shear stress and KATP channel dependent endothelial cell proliferation. FASEB J., 2004, 18, A842.

Hawkins, B. J., Jenkins, B., Chatterjee S. and Fisher, A.B. Transcription factor alterations in response to shear stress cessation in flow-adapted pulmonary microvascular endothelial cells. FASEB J., 2004, 18, A842.

Chatterjee, S.,  Flow adaptation in Lung endothelia. Proccedings of the Grover Conference on the Pulmonary Circulation: Genetic and Environmental Determinants of Pulmonary Endothelial Cell Function Vol 3, 2004.

T.Milovanova, Feinstein, S.I., Chatterjee, S., Hawkins, B.J., Kotelnikova, I.N., DeBolt, K., Muniswamy, M.,  Moore J.S., and Fisher, A.B., Proliferation of lung endothelial cells with abrupt reduction in shear stress requires reactive oxygen species, generated by NADPH oxidase. FASEB J. 2005, 19, A1208

B.J. Hawkins, S. Chatterjee, M. Muniswamy, C.J. Kirkpatrick and A. Fisher, The response of human pulmonary microvascular endothelial cells to flow-cessation following shear stress adaptation. FASEB J. 2005, 19, A1231.

Chatterjee, S., An in vitro flow adaptation chamber replaces animals in an ischemia/ reperfusion model to study oxidant generation. 5th World Congress on Alternatives & Animal Use in the Life Science Report, 2005.  Chatterjee, S., Levitan, I. and Fisher, A.B. The KATP channel is an important component of flow sensing in the pulmonary microvasculature. FASEB J. 2006, 20, 1261.

Milovanova, T., Muniswamy, M., Chatterjee, S., Hawkins, B.J., DeBolt, K., Moore, J.S., Fisher, A.B., Caveolin-1 knockout lung endothelial cells. FASEB J. 2006,20, 1455.  

 

Invited Talks and Seminars

 

1. An in vitro flow adaptation chamber replaces animals in an ischemia/ reperfusion model to study oxidant generation. 5th World Congress on Alternatives & Animal Use in the Life Science, August 21-25, Estrel Convention Center, Berlin. Germany, 2005.

 

2. Flow adaptation in Lung endothelia. Invited talk at the 13th Grover Conference on the Pulmonary Circulation: Genetic and Environmental Determinants of Pulmonary Endothelial Cell Function, Lost Valley Ranch and Conference Center, Sedalia, CO. Sept 9-12, 2004.

 

3. Shear stress, KATP channel and ischemia. Invited talk at III rd annual conference of Society of Free radical research (India), Chidambaram, India, Feb 10-12, 2004.  

 

4. KATP channels and oxidant generation in the pulmonary microvasculature. Invited talk at the International Conference on the role of free radicals in health and disease and IInd annual conference of Society of Free radical research (India), Lucknow, India, Feb 10-12, 2003.  

RESEARCH SUPPORT
ACTIVE

 

Endothelial Generation of ROS in Lung Ischemia

PI:  Aron B. Fisher

Role on Project:  Research Assistant Professor

Agency:  NIH/NHLBI

Type:  R01-HL075587

 

 

Major Goals:

1.     Identify endothelial cell membrane ion channels responsible for ischemia-mediated membrane depolarization.

2.     Determine endothelial ROS generator with ischemia.

3.     Identify cell membrane Ca2+ channels responsible for endothelial cell Ca2+ influx with ischemia.

4.     Identify transcription factors that are activated with ischemia and define parameters for subsequent cell division.

 

An in vivo flow adaptation chamber replaces animals in a ischemia/reperfusion model to study oxidant injury.

PI: Shampa Chatterjee, Ph.D.

Agency: Johns Hopkins University (Center for Alternatives to Animal Testing)

Major Goals:

  1. ROS generation with simulated I/R in a flow chamber using endothelial cells isolated from rats and mice. 
  2. To examine the changes in ROS generation in the above mentioned I/R model using pharmacological agents.
  3. Endothelial cells lacking KATP will be compared for I/R responses in terms of ROS generation and Ca2+ signaling.

 

Role of NADPH oxidase derived reactive oxygen species (ROS) in ventilation induced vascular permeability in the lung

PI: Shampa Chatterjee, Ph.D.

Agency: Mccabe Foundation

Major Goals:

1. Monitoring the ROS generation in an in situ isolated perfused lung model with high and low PIP.

2. Measuring the microvascular permeability and edema in isolated perfused mouse lungs.